Coding

Part:BBa_K4594012:Design

Designed by: Shubai Wang   Group: iGEM23_ShanghaiTech-China   (2023-10-01)


PDHC


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 287
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 442
    Illegal PstI site found at 287
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 287
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 287
    Illegal NgoMIV site found at 183
    Illegal AgeI site found at 561
    Illegal AgeI site found at 1102
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Source

The coding sequence of PDHC comes from the genome of Synechococcus elongatus PCC7942

References

[1] Hirokawa Y, Kubo T, Soma Y, Saruta F, Hanai T. Enhancement of acetyl-CoA flux for photosynthetic chemical production by pyruvate dehydrogenase complex overexpression in Synechococcus elongatus PCC 7942. Metab Eng. 2020 Jan;57:23-30. doi: 10.1016/j.ymben.2019.07.012. Epub 2019 Aug 1. PMID: 31377410.